Regulatory
Part:BBa_K4613006
Designed by: Qinfei Zhou Group: iGEM23_NAU-CHINA (2023-10-09)
Ptra
TraR protein forms a complex with its signaling molecule. This complex binds to the Ptra promoter, increasing the rate of transcription. Compared with BBa_K553002, we have truncated the sequence to 76 bp, including the tra box II、-35 sequences、-10 sequences and an RBS.
Reference
- White C E, Winans S C.Identification of amino acid residues of the Agrobacterium tumefaciens quorum-sensing regulator TraR that are critical for positive control of transcription[J].Mol Microbiol,2005, 55 (5): 1473-86.
- Fuqua C, Winans S C.Conserved cis-acting promoter elements are required for density-dependent transcription of Agrobacterium tumefaciens conjugal transfer genes[J].J Bacteriol,1996, 178 (2): 435-40.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 14
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |